High resolution analysis of anti-migratory inhibitors in high grade glioma treatment

IntroductionGlioblastoma Multiforme (GBM) is an extremely aggressive form of glioma tumour. The characteristically invasive properties GBM contribute to the poor prognosis in suffers. This study focuses on therapeutic potential four anti-migratory drugs - 6-bromo- indirubin-3’oxime (BIO), CCG-1423, Latrunculin A (LAT A) and Lithium Chloride (LiCl), vitro. migration distances migrating cells cell circularity for each treatment untreated control were measured statistically analysed.MethodsGBM spheroids U251 line treated with BIO, LAT or LiCl plus preparation this study. These sectioned using immunohistochemical techniques scanned prior QuPath. Cell was from perimeter tumour spheroid edge cell. obtained ‘cell index’ function. separately within spheroid. repeated section data analysed SPSS.ResultsEach treatments showed significant statistical differences compared distance measurements. shown have greatest effect decreasing distances. analysis indicated that migrated significantly more rounded than treatment. those across treatments.ConclusionThe suggests further study, analyse effects these vivo, as they demonstrated cells. should be amended accurately represent changes morphology during migration.